DNA

Part:BBa_K5319676:Design

Designed by: Wang Xuanhao   Group: iGEM24_ZJU-China   (2024-10-01)


λ peptide-GS linker-vioB


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 90
    Illegal PstI site found at 775
    Illegal PstI site found at 2017
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 775
    Illegal PstI site found at 2017
    Illegal NotI site found at 992
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 2077
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 90
    Illegal PstI site found at 775
    Illegal PstI site found at 2017
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 90
    Illegal PstI site found at 775
    Illegal PstI site found at 2017
    Illegal NgoMIV site found at 931
    Illegal NgoMIV site found at 1845
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The binding protein skeleton binds together multiple enzymes with λ peptide-GS linker.


Source

vioB is from the NCBI library

References